Microbiology

LECTURES

1. Introduction to Microbiology- Macromolecules of microorganisms

2. Microscopy and cell morphology. Cell membranes and cell walls.

3. Movement of microorganisms. Surface structures and encapsulation of prokaryotes.

4. Microbial nutrition and laboratory cultures.

5. Metabolism of microorganisms.

6. Microbial growth. Environmental effects on microbial growth.

7. Overview of genes and gene expression.

8. Regulation of Gene Expression.

9. Microbial development and systematics.

10. Principles of Microbial Ecology.

11. Synthesis and processing of RNA.

12. Mechanisms of transfer and exchange of genetic material. Transposons & Plasmids.

13. Viruses, proliferation, viral diversity.

PRACTICAL EXERCISES

Practical 1: Introduction to a microbiology laboratory and safety

Practical 2: Preparation and sterilization of nutrients – use of autoclave

Practical 3: Aseptic methods in microbiology

Practical 4: Serial dilutions and microbial growth

Practical 5: Pure Cultures - Growth of bacteria in liquid cultures

Practical 6: Microscopic examination of microorganisms

Practical 7: Microbiological analysis of water

Practical 8: Gram staining