Molecular Diagnostics and Epidemiology

Lectures

Lesson 1: Introduction and history of diagnosis.

Lesson 2: Diseases - infectious, physiological and metabolic errors, genetic basis of diseases, hereditary diseases.

Lesson 3: Infection - mode of transmission in infections, factors predisposing to microbial pathogenesis, types of infectious diseases - bacterial, viral, fungal, protozoan and other parasites.

Lesson 4: Philosophy and general approach to clinical specimens, Specimen collection - method of specimen collection, transport and processing.

Lesson 5: Normal microbial flora of the human body, host-parasite relationships.

Lesson 6: Traditional methods and tools of disease diagnosis: diagnosis of infection caused by Streptococcus, Coliforms, Salmonella, Shigella, Vibrio and Mycobacterium.

Lesson 7: Diagnosis of fungal infections. Important fungal diseases

Lesson 8: Diagnosis of DNA and RNA viruses.

Lesson 9: Diagnosis of protozoan diseases.

Lesson 10: Molecular Diagnostic Techniques I

Lesson 11: Molecular Diagnostic Techniques II

Lesson 12: Genomics and Metagenomics as diagnostic tools

Lesson 13: Microarrays as diagnostic tools

Laboratory exercises

1. Polymerase Chain Reaction.

2. Real-time Polymerase Chain Reaction.

3. Applications of microarrays

4. Hybridization against Southern

5. Hybridization by Northern

6. Isolation and characterization of proteins

7.ELISA